单色显微镜数码相机DS-Qi2

弱荧光拍摄与宽视野

DS-Qi2单色相机具备高像素密度、高灵敏度与低噪声的特性。

 

 超大规格CMOS传感器

       继承自专业数码单反相机并由尼康制造的CMOS传感器和成像技术，经过优化现已用于显微技术。

高灵敏度  可检测微弱荧光信号

       7.3um像素尺寸，高量子效率配合低读出噪声使DS-Qi2甚至可以检测微弱的荧光信号。

的线性特性 

      可实现可靠的定量分析

       ± 1%的线性误差，DS-Qi2是荧光强度测量的绝好工具。无论时间强度测量还是比率测量都可轻松应对。

  

 
Indian Muntjac Deer Skin Fibroblast Cells, Cytoskeletal F-actin labeled with Alexa Fluor 488

Sample courtesy of: Michael Davidson and Florida State University

 高帧率  

      即使使用荧光图像亦可实现快速对焦

      2.2个电子读出噪声配合大容量像素及0.6电子暗电流使其可以拍摄极小噪声的荧光图像。

低噪声 

      获取微弱荧光的极低噪声图像

        2.2个电子读出噪声配合大容量像素，是获取的荧光图像具有极低的噪声和的动态范围。

  

   
 LLC-PK1 cells expressing GFP-EB3 tubulin Low noise and large linear full well capacity allows the acquisition of large dynamic range in a single capture.
A captured image is shown intensity scaled to show both the brightest and dimmest areas.
Sample courtesy of: Michael Davidson, National High Magnetic Field Laboratory, Florida State University

时间序列成像    

      使用NIS-Elements软件进行荧光时间序列成像

       结合宽视野和高像素密度及低噪声，DS-Qi2是时间序列拍摄应用的选择。 

  
Time-lapse images (every 1 second) of LLC-PK1 cells with GFP-EB3 tubulin. Each image represents the maximum intensity projection of the timelapse, allowing visualization of the end-binding protein located on the microtubule plus-ends, and allowing tracing of the microtubule path.
DS-Qi2 captures an extremely large field of view, but still represents very fine details as demonstrated in this cropped timelapse sequence from a large FOV image.
Objective: CFI Plan Apochromat λ 60x oil / NA: 1.4)
Sample courtesy of: Michael Davidson, National High Magnetic Field Laboratory, Florida State University